Biomat on microfluidic chip

Programme 2014 - 2020 INTERREG V-A Belgium - The Netherlands (Vlaanderen - Nederland)

Date of latest update: 2022-08-15

Description

Description (NL):

Description (EN):

The treatment of patients will in the future be increasingly focused on 'personal regenerative medicine'. This is a new form of medicine in which doctors treat patients by creating new skin, bone or even an organ from cultured cells and biomaterials. This form of medicine generates great expectations in terms of innovative and personal care, but also has major challenges because it requires much high-quality knowledge and technology. Both Belgium and the Netherlands want to develop a knowledge economy to find answers to new challenges and at the same time to sustainably strengthen employment in the region. Platforms such as the Flemish RegMed and the Dutch Brightlands already support new developments within healthcare. To be able to test biomaterials on a large scale, a so-called 'organ-on-chip' offers new possibilities. These are small plates that mimic an organ or tissue and on which new biomaterials can be tested immediately in a physical environment. To develop this chip and prepare it for a broad introduction and use, a strong partnership is required consisting of both academic and industrial partners. 'Biomat' therefore creates a brand new microfluidic chip: the 'organ-on-chip 2.0'. This chip is seeded with stem cells to mimic a body's own organ or tissue, provided with supply and discharge of micro-fluid and sensitive sensors to perform measurements. The innovative aspect of this chip is the three-dimensional (3D) environment that is formed, in contrast to classic cell culture plates in which cells or tissues grow on a flat surface. Moreover, microfluidics - very small channels through which liquids are transported - mimic the transport of bodily fluids. To demonstrate the functionality of this revolutionary technology, examples will be constructed based on three types of clinical applications: bone, muscle and on-chip vascularized bone or muscle.

Achievements (NL):

De behandeling van patiënten zal zich in de toekomst steeds meer richten op 'persoonlijke regeneratieve geneeskunde'. Dit is een nieuwe vorm van geneeskunde waarbinnen artsen patiënten behandelen door nieuwe huid, bot of zelfs een orgaan op te bouwen uit gekweekte cellen en biomaterialen. Deze vorm van geneeskunde wekt grote verwachtingen wat betreft innovatieve en persoonlijke zorg, maar kent ook grote uitdagingen omdat het veel hoogwaardige kennis en techniek vereist. Zowel België als Nederland willen hierin een kenniseconomie ontwikkelen. Platformen zoals het Vlaamse RegMed en het Nederlandse Brightlands ondersteunen reeds nieuwe ontwikkelingen binnen de gezondheidszorg. Om biomaterialen op grote schaal te kunnen testen, biedt een zogeheten 'orgaan-op-chip' nieuwe mogelijkheden. Dit zijn kleine plaatjes die een orgaan of weefsel nabootsen en waarop nieuwe biomaterialen direct kunnen worden getest in een lichaamsechte omgeving. Om deze chip te ontwikkelen en gereed te maken voor een brede introductie en gebruik, is een sterk partnerschap nodig van zowel academische als industriële partners. ‘Biomat’ creëert daarom een gloednieuwe microfluïdische chip: de 'orgaan-op-chip 2.0'. Deze chip wordt ingezaaid met stamcellen om een lichaamseigen orgaan of weefsel na te bootsen, voorzien van aan- en afvoer van micro-vloeistof en gevoelige sensoren om metingen te verrichten. Het vernieuwende aspect van deze chip is de 3D-omgeving die wordt gevormd, in tegenstelling tot klassieke celkweekplaten waarin cellen of weefsels op een vlak oppervlak groeien. Bovendien wordt door middel van microfluïdica - zeer kleine kanaaltjes waarin vloeistoffen worden vervoerd - het transport van lichaamsvloeistoffen nagebootst. Om de functionaliteit van deze revolutionaire technologie aan te tonen, zullen demonstrators worden gebouwd op basis van drie soorten klinische toepassingen: bot, spier en op-chip gevasculariseerde bot of spier. In de eerste twee jaar van het project zijn er belangrijke stappen gemaakt richting de ‘orgaan-op-chip 2.0’, door verschillende technische en biologische technologieën te ontwikkelen. Eerste ontwerpen en modellen van de chip zijn gemaakt. De ontwikkelde inzetstukken zijn geschikt gebleken voor het vormen van driedimensionale constructen van patiënt-eigen cellen en verschillende biomaterialen op microschaal. Daarnaast hebben we meerdere robuuste bioassays ontwikkeld voor het screenen van de toxiciteit/biocompatibiliteit en de functionaliteit van nieuw ontwikkelde biomaterialen. Voor het omhulsel van de chip, dat de constructen moet voorzien van voedingsstoffen, zijn ook verschillende ontwerpen getest, maar er wordt nog steeds gezocht naar de juiste combinatie van materialen en technieken om de benodigde resolutie te verkrijgen. Eerste succesvolle experimenten met impedantie sensoren, die de cellen en het vormen en verwijderen van weefsel kunnen meten, zijn uitgevoerd. De massa spectrometrische methode is verder gevalideerd om specifiek eluerende moleculen te kunnen bepalen op zeer laag niveau. Terwijl de chips getest zijn met cellijnen en primaire cellen van patiënten, is er ook veel werk gedaan aan het ontwikkelen van protocollen voor het differentiëren van humane geïnduceerde pluripotente stamcellen (hIPSCs) richting spierweefsel en botweefsel. Daarnaast zijn deze cellen voorzien van reporters waarmee hun groei en metabolisme niet-invasief gevolgd kunnen worden. Vorming van 3D microweefsels en organoiden met behulp van deze cellen is aangetoond. Ook is de ontwikkeling van het platform voor vascularisatie van microweefsels, Organo®Graft Plate vergevorderd. In de synthese en selectie van geschikte technieken om microschaal monsters van biomaterialen te maken, zijn belangrijke stappen gemaakt. Samenvattend, op alle aspecten van het project is voortgang geboekt. Integratie van verschillende componenten in de chip is ook gestart. De resultaten laten zien dat de iteratieve optimalisatie van verschillende onderdelen nodig is om demonstrators voor verschillende toepassingen te ontwikkelen en daarom zullen alle activiteiten in het project in verschillende mate gecontinueerd worden.

Achievements (EN):

In the future, the treatment of patients will increasingly focus on ‘personal regenerative medicine’. This is a new form of medicine in which doctors treat patients by building new skin, bone or even an organ from cultured cells and biomaterials. This form of medicine raises high expectations in terms of innovative and personal care, but also has major challenges because it requires a lot of high-quality knowledge and technology. Both Belgium and the Netherlands want to develop a knowledge-based economy. Platforms such as the Flemish RegMed and the Dutch Brightlands already support new developments in healthcare. In order to be able to test biomaterials on a large scale, a so-called ‘organ-on-chip’ offers new possibilities. These are small plates that mimic an organ or tissue and on which new biomaterials can be tested directly in a body-like environment. To develop and prepare this chip for a broad introduction and use, a strong partnership is needed from both academic and industrial partners. ‘BioMat’ therefore creates a brand new microfluidic chip: the “organ-on-chip 2.0”. This chip is sown with stem cells to mimic a body’s own organ or tissue, provided with the supply and discharge of micro-liquid and sensitive sensors to perform measurements. The innovative aspect of this chip is the 3D environment that is formed, unlike classic cell culture plates in which cells or tissues grow on a flat surface. In addition, microfluidica — very small channels in which liquids are transported — simulates the transport of body fluids. To demonstrate the functionality of this revolutionary technology, demonstrators will be built based on three types of clinical applications: bone, muscle and on-chip vascularised bone or muscle. In the first two years of the project, important steps have been made towards the “organ-on-chip 2.0” by developing various technical and biological technologies. First designs and models of the chip were made. The developed inserts have proven to be suitable for forming three-dimensional constructs of patient-own cells and various microscale biomaterials. In addition, we have developed several robust bioassays to screen the toxicity/biocompatibility and functionality of newly developed biomaterials. For the shell of the chip, which must provide the constructs with nutrients, various designs have also been tested, but the right combination of materials and techniques is still being sought to obtain the required resolution. First successful experiments with impedance sensors, which can measure the cells and the formation and removal of tissue, have been carried out. The mass spectrometric method has been further validated to determine specifically eluating molecules at very low levels. While the chips have been tested with cell lines and primary cells of patients, much work has also been done to develop protocols for differentiating human-induced pluripotent stem cells (hiPSCs) towards muscle tissue and bone tissue. In addition, these cells are provided with reporters that allow their growth and metabolism to be monitored non-invasively. Formation of 3D microtissues and organoids using these cells has been demonstrated. The development of the platform for vascularisation of microtissues, Organo®Graft Plate, is also advanced. In the synthesis and selection of appropriate techniques to make microscale samples of biomaterials, important steps have been made. In summary, progress has been made on all aspects of the project. Integration of different components into the chip has also started. The results show that the iterative optimisation of different parts is needed to develop demonstrators for different applications and therefore all activities in the project will be continued to varying degrees.

Website: http://www.grensregio.eu/

Thematic information

Health and social services New products and services

Specific Objective: Innovation of products/ services/ applications/ processes in the form of industrial research and experimental development through the cooperation among enterprises as well as among enterprises, R&I centers and universities

Thematic Objective: (01) strengthening research, technological development and innovation by:

Investment Priority:

(01b) promoting business investment in R&I, developing links and synergies between enterprises, research and development centres and the higher education sector, in particular promoting investment in product and service development, technology transfer, social innovation, eco-innovation, public service applications, demand stimulation, networking, clusters and open innovation through smart specialisation, and supporting technological and applied research, pilot lines, early product validation actions, advanced manufacturing capabilities and first production, in particular in key enabling technologies and diffusion of general purpose technologies

Partners (8)

Lead Partner: Universiteit Maastricht

Address: Minderbroedersberg 4-6, 6200 MD Maastricht, Netherlands

Legal status: public

Total budget: EUR 1 837 045.33

ERDF budget: EUR 918 522.66

Interuniversitair Micro-Elektronica Centrum - IMEC

Name: Interuniversitair Micro-Elektronica Centrum - IMEC

Address: Wetenschapspark 1, 3590 Diepenbeek, Belgium

Legal status: private

Total budget: EUR 346 914.25

ERDF budget: EUR 173 457.13

Xplore Instruments B.V.

Name: Xplore Instruments B.V.

Address: Arendstraat 5, 6135 KT Sittard, Netherlands

Legal status: private

Total budget: EUR 14 048.63

ERDF budget: EUR 7 024.32

Tenco DDM

Name: Tenco DDM

Address: Seinhuisstraat 3 bus 8, 3600 Genk, Belgium

Legal status: private

Total budget: EUR 201 397.27

ERDF budget: EUR 100 698.64

Mimetas B.V.

Name: Mimetas B.V.

Address: J.H. Oortweg 19, 2333 CH Leiden, Netherlands

Legal status: private

Total budget: EUR 800 000.00

ERDF budget: EUR 400 000.00

Leids Universitair Medisch Centrum

Name: Leids Universitair Medisch Centrum

Address: Albinusdreef 2, 2333 ZA Leiden, Netherlands

Legal status: public

Total budget: EUR 450 854.96

ERDF budget: EUR 225 427.48

Katholieke Universiteit Leuven

Name: Katholieke Universiteit Leuven

Address: Oude Markt 13, 3000 Leuven, Belgium

Legal status: public

Total budget: EUR 554 795.66

ERDF budget: EUR 277 397.83

DSM Materials Science Center B.V.

Name: DSM Materials Science Center B.V.

Address: Urmonderbaan 22, 6167RD Geleen, Netherlands

Legal status: private

Total budget: EUR 294 943.90

ERDF budget: EUR 147 471.95

Partners map

Partners and Projects info

Interuniversitair Micro-Elektronica Centrum - IMEC

Biomat on microfluidic chip

Partners and Projects info

Xplore Instruments B.V.

Biomat on microfluidic chip

Partners and Projects info

Universiteit Maastricht

Biomat on microfluidic chip

Partners and Projects info

Tenco DDM

Biomat on microfluidic chip

Partners and Projects info

Mimetas B.V.

Biomat on microfluidic chip

Partners and Projects info

Leids Universitair Medisch Centrum

Biomat on microfluidic chip

Partners and Projects info

Katholieke Universiteit Leuven

Biomat on microfluidic chip

Partners and Projects info

DSM Materials Science Center B.V.

Biomat on microfluidic chip

Lead partner

Project partner

Summary

Project name (EN): Biomat on microfluidic chip

Project acronym: Biomat

Project start date: 2018-01-01

Project end date: 2021-07-31

Project status: closed

Total budget/expenditure: EUR 4 500 000.00

Total EU funding (amount): EUR 2 250 000.00

Co-financing sources:

ERDF: Amount, EUR 2 250 000.00.

Documents

Documents on this project are listed below, and organised by category. Please note field "Data source", attached to each document. Keep.eu users are free to use these documents as long as they mention the data source and keep.eu.

Videos / films

Biomat on microfluidic chip

Attachment type: link

Type of document: Video / film

Data source: https://www.grensregio.eu/projecten/biomat-on-microfluidic-chip

Publication date: 2021-12-21

Document date: 2021-03-01

Languages: nl

Information regarding the data in keep.eu on the programme financing this project

Financing programme

2014 - 2020 INTERREG V-A Belgium - The Netherlands (Vlaanderen - Nederland)

Last month that data in keep.eu was retrieved from the Programme's website or received from the Programme

2022-08-25

No. of projects in keep.eu / Total no. of projects (% of projects in keep.eu)

94 / 94 (100%)

No. of project partnerships in keep.eu / Total no. of project partnerships (% of project partnerships in keep.eu)

1 226 / 1 226 (100%)

Notes on the data

Information provided by the Programme. Achievements were retrieved by Interact from the Programme website (http://www.grensregio.eu/en), or a website directly linked from it, on 2021-11-04, 2023-01-12 and 2024-02-24.

Fields project name, description and achievement (from 61 projects) translated from Dutch into English using the European Commission's eTranslation machine translation service.

The projects which fields include an eTranslation English text are the ones with the following acronyms: Beton naar hoogwaardig beton, Biomat, Blijf aan Z, Circulair onderhoud, CO2 voor energieopslag (EnOp), De blauwe keten, De Lerende Euregio Scheldemond, Deeldezon, Fotonica pilootlijnen, Grensinfovoorziening VL-NL, Grenspark Groot Saeftinghe, Grenzeloos Biobased Onderwijs, Intelligenter Fruit Telen, Lerend Netwerk Biobouwers, Leve(n) de Bodem, LogistiekLerenZonderGrenzen, Meer natuur voor pittig fruit, Praktijklab Corrosie&Isolatie, REVIVAK (Revival vakmanschap), Entomospeed, PV OpMaat, ENLEB, Smart tooling procesindustrie, 2B Connect, Accelerate³, AQUA-VLAN 2, BIO – HarT, CrossCare, IMPAKT!, GIST, Educavia, CrossRoads 2, IMPROVED, i-4-1-Health, DEMI MORE, Meer natuur voor pittig fruit, Train4SmartServices, Link2innovate, Nano4Sports, See2Do!, eco2eco, GrasGoed – NGAG, Growing a Green Future, SKiN-HUID, Smartsediment, Smart Services Bridge, Trans Tech Diagnostics, Triple F (Food From Food), ZORO, ZULU, Glitch, Intelligenter Fruit Telen, I-QUA, PASSAnT, RHEDCOOP, TERTS, Waterstofregio 2.0 and Werkinzicht.

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Contact Form

Biomat on microfluidic chip

Description

Thematic information

Partners (8)

Partners map

Summary

Documents

Information regarding the data in keep.eu on the programme financing this project

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